加入收藏
百奥莱博公司客服电话百奥莱博QQ百奥莱博QQ百奥莱博QQ
百奥莱博公司微信服务号

产品目录

Paxillin/PXN抗体图片
产品货号:
Z23918
中文名称:
Paxillin/PXN抗体
英文名称:
Anti-Paxillin/PXN Antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称Anti-Paxillin/PXN Antibody
指标别名paxillin;PXN
克隆性Polyclonal
检验物种human,mouse,rat
应用范围WB,IHC,IF,IHC-F,ICC/IF,FCM
基因名称PXN
抗体来源Rabbit
抗体类型IgG
免疫原A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin(456-472aa HEKDGKAYCRKDYFDMF),identical to the related mouse and rat sequences.
实际分子量65KD
成分500μg/mL antibody with PBS,0.02% NaN3,1mg BSA
纯化方式Immunogen affinity purified.
浓度500μg/mL
产品形态溶液
保存条件12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
背景资料PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge.Salgia et al.(1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions,it is presumed through a direct association with the cytoplasmic tail of beta-integrin.The N-terminal region of paxillin is rich in protein–protein interaction sites.The proteins that bind to paxillin are diverse and include protein tyrosine kinases,such as Src and FAK,structural proteins,such as vinculin and actopaxin,and regulators of actin organization,such as COOL/PIX and PKL/GIT.Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation,creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains,a proline-rich domain containing a consensus SH3-binding site,and 3 potential SH2-binding sites.On Northern blots,paxillin was expressed as a 3.7-kb mRNA in all tissues tested.
Uniprot IDP49023
推荐配套试剂Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。
推荐稀释比
Western blot (WB) 1:500~2000
Immunohistochemistry in paraffin section (IHC) 1:50~400
Immunohistochemistry in frozen section (IHC-F) 1:50~400
Immunofluorescence (IF) 1:50~400
Immunocytochemistry/Immunofluorescence(ICC/IF) 1:50~400
Flow cytometry (FCM) 1~3μg/1×106 cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

相关搜索:Paxillin/PXN抗体Paxillin抗体PXN多抗Anti-Paxillin/PXN Antibody
首页 |  关于我们 |  联系我们 |  在线咨询 |  网站地图
北京百奥莱博科技有限公司 京ICP备14007103号-3